citrate buffer (Thermo Fisher)

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Thermo Fisher citrate buffer
Citrate Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/citrate+buffer/Citrate+Buffer/10__22203_slash_ecm__v033a11-128-23-28
Average 99 stars, based on 1 article reviews

citrate buffer - by Bioz Stars, 2026-09

99/100 stars

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Purification:

Article Title: Stereochemical identity of lipid nanoparticles modulates protein expression via internal lipid organization
Article Snippet: TriLink CleanCap® 5-methoxyuridine (5moU) modified EGFP-mRNA and CleanCap® AG Reagent were purchased from Tebubio (Heerhugowaard, The Netherlands). .. Quant-ITTM RiboGreen RNA Assay Kit, 0.5 M Citrate Buffer (pH 4.0), Invitrogen reagents for IVT reactions, IVT purification, and agarose gel staining, and Gibco supplements for culture media were purchased from Fisher Emergo B.V. (Landsmeer, The Netherlands). .. DPBS (10×) was obtained from VWR International B.V. (Amsterdam, The Netherlands).

Agarose Gel Electrophoresis:

Staining:

Article Title: RNA aptamers and uses thereof
Article Snippet: .. Antigen retrieval was performed with Citrate buffer (pH6 Thermo-Fisher) by microwaving the tissue for 30 minutes at 100 W. Tissues incubated in PBS O/N at RT, incubated with dextran sulfate sodium/PBS (1:2 m/V—Pharmacia biotech) for 30′ washed with PBS, and then stained with Cy3-labelled aptamer (10 ug/ml) in PBS for 30′, masked with 2% BSA, and counterstained with antibodies and/or DAPI. .. Whole stained tissue sections were scanned at 20× with the Olympus VS120 equipped with DAPI CUBE 455 nm (12.941 ms), a FITC CUBE 518 nm (410.271 ms) and a TRITC CUBE 580 nm (592.6 ms).

Biomarker Discovery:

Article Title: The WEE1 inhibitor azenosertib broadly enhances efficacy of antibody-drug conjugates with topoisomerase I and microtubule inhibitor payloads
Article Snippet: Blocks were cut into 5 μm sections using a Leica RM2135 microtome. .. For PD biomarker analysis, sections were dewaxed and rehydrated in distilled water before antigen retrieval with 200 mL citrate buffer (pH 6.0) and heating for 10 min. Once the citrate buffer cooled, slides were removed and placed into an autostainer (ThermoFisher, Waltham, MA). ..

Incubation:

Article Title: Exogenous testosterone exacerbates pre-neoplastic lesions in the prostate of NKX3.1-deficient mice.
Article Snippet: .. After antigen retrieval using citrate buffer (pH = 6.0), sections were blocked with 5% normal goat serum at room temperature for 30 minutes and incubated overnight at 4°C with an anti-p53 primary antibody (Thermo Fisher Scientific, Waltham, MA, USA). .. The sections were then incubated with an Alexa Fluor 488-conjugated secondary antibody (Thermo Fisher Scientific).

Article Title: Integrative Multi‐Omics Analysis Elucidates the Progressive Disease Landscape and Reveals Dynamic Protein Biomarkers for <scp>MASLD</scp> Surveillance
Article Snippet: .. Sections were deparaffinized, rehydrated, antigen- retrieved with citrate buffer (pH 6.0, 95°C, 15 min), and blocked with 5% bovine serum albumin (BSA) at room temperature for 60 min. Primary antibodies specific for AKR1B10 (Thermo Fisher, PA5- 22036, 1:1000), secreted phosphoprotein 1 (SPP1; Affinity, AF0227, 1:1000), COL1A2 (Affinity, AF7001, 1:1000), and CYP2C19 (Proteintech, 55 453–1- AP, 1:3000) were incubated overnight at 4°C. ..

Article Title: Calcium-modulated GJB6-GRHL3 positive feedback loop attenuates ESCC progression through AKT signaling pathway inhibition.
Article Snippet: .. To block endogenous peroxidase activity, 0.3% H2O2 was applied to incubate the slices for 30 min. After the antigen retrieval in citrate buffer pH 6.0 for 15 min and rinsing three times with PBS, sections were incubated with the first antibodies overnight at 4°C [GJB6 (Invitrogen, California, USA), GRHL3 (Aviva Systems Biology, California, USA), Ki-67 (Abcam, Cambridge, UK), p-AKT (Cell Signaling Technology, Boston, USA)]. ..

Article Title: A chlorhexidine-releasing epoxy-based coating on titanium implants prevents Staphylococcus aureus experimental biomaterial-associated infection
Article Snippet: .. The sections were subjected to heatinduced epitope retrieval (HIER) (van der Loos, 2010), performed in a PreTreatment Module (Lab Vision) by incubation in citrate buffer pH 6.0 (TA-250-PM1X, Thermo Fisher Scientific) for 20 min at 98 °C, rinsed with tap water and incubated with Superblock (AAA999, Klinipath) for 10 min at RT. .. Sections were incubated overnight at 4 °C with rat anti-mouse monoclonal F4/80 (macrophages; MCA497GA, clone: CI:A3-1, Serotec) diluted 1: 1000 in Tris-HCl-buffered saline (TBS; 50 mM Tris, 0.9 % NaCl), subsequently with rabbit anti-rat IgG (6130-01, fab2; SBA/ITK) diluted 1: 3000 in TBS with 20 % normal mouse serum for 30 min at RT, followed by undiluted BrightVision anti-rabbit AP (DPVB-55AP, Immunologic) for 30 min at RT and finally with Vector Blue (SK-5300, Vector Labs) for 10 min at RT, with three TBS washes between all incubations.

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citrate buffer (Thermo Fisher)

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